A lot of science research is freely available. See below for some good websites to help locate these resources:
Bar Code of Life Data Systems (BOLD) is an online workbench that aids collection, management, analysis, and use of DNA barcodes. It consists of 3 components (MAS, IDS, and ECS) that each address the needs of various groups in the barcoding community.
Bar Code of Life Data Systems (BOLD) Identification Engine The BOLD Identification System (IDS) accepts sequences from the 5' region of the mitochondrial gene COI and returns a species-level identification when one is
possible. Further validation with independent genetic markers will be desirable in some forensic applications.
EMBL - European Bioinformatics Institute
Entrez databases from NCBI
Institute Pasteur - Biological Databases
National Center for Biotechnological Information (NCBI) from National Library of Medicine (includes GeneBank, Entrez, Cancer Genome Database, Human Genome Database, etc.)
National Center for Biotechnology Information (NCBI) Handbook
OMIM, Online Mendelian Inheritance in Man
Comparative Gene Finding
ECR-Browser.
eShadow.
zPicture.
UCSC genome browser. -- Current Annotations for the Genomic DNA Sequences
Human Genome Project (HGP) coordinated by the U.S> Dept. of Energy and the National Institutes of Health.
National Institutes of Health (NIH). Roadmap for Medical Research. Epigenomics Program.
National Institutes of Health (NIH) Roadmap for Medical Research. Structural Biology.
Protein Data Bank An Information Portal to Biological Macromolecular Structures from the Research Collaboratory for Structural Bioinformatics (RSCB). "The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies ... [and can be used to do] ... simple and advanced searches based on annotations relating to sequence, structure and function" Summaries include parameters, unit cell, classification, classification, images and visulaization KiNG, Jmol, WebMol, Rasmol, etc.), and more. Each structure summary includes PubMed's Medical Subject Headings (MeSH) !!
Genetics is the study of inheritance. First studied by Gregor Mendel in 1850-1870 using peas.
Mendels genetic factors proved in 1952 to be DNA. DNA is divided into genes : each gene has the code for making one protein. Human DNA has roughly 25,000 genes.
Genes can be several alternative types called alleles example : allele B for brown hair, or b for blond. You inherit two alleles (one from each parent). A Dominant allele has an effect whether you have one copy of it, or two copies. eg BB or Bb (brown hair).
A Recessive allele only has an effect if you inherit two copies. eg bb (blond).
Genotype is the genetic makeup of an individual eg Bb. Phenotype is the physical appearance eg brown hair.
Genotype | Phenotype | |
---|---|---|
T T | Homozygous dominant | Tall plant |
T t | Heterozygous | Tall plant |
t t | Homozygous recessive | Short plant |
1) Different characteristics are caused by different alleles.
2) An organism inherits two alleles for each trait.
3) Each gamete only contains one allele for each trait.
4) In a heterozygous individual, only the dominant allele affects the trait.
Monohybrid cross - only one trait is studied ( eg black/brown fur).
Dihybrid cross - two traits are studied ( black/brown fur and short/long hair).
Trait | Dominant | Recessive |
---|---|---|
Flower color | Purple | White |
Flower position | Axial | Terminal |
Seed color | Yellow | Green |
Seed shape | Round | Wrinkled |
Pod color | Green | Yellow |
Plant height | Tall | Dwarf |
Test cross
A test cross is used to work out an unknown genotype ( dominant phenotype ). The unknown is crossed with a homozygous recessive individual. If all the offspring have the dominant phenotype, the unknown parent must be homozygous dominant.
If some of the offspring have the recessive phenotype, the unknown parent must be heterozygous.
Dihybrid cross
Each trait is inherited independently. There are four possible phenotypes in the offspring.
If two heterozygous individuals are crossed ( eg BbSs : black, short-haired guinea pigs ) the result is a ratio of :
9 : 3 : 3 : 1 black, black brown brown short hair long-hair short-hair long-hair
Probability
Punnett squares work out the probability of getting a particular offspring. For example, if two heterozygous individuals breed, 3/4 of the offspring will have the dominant trait, and 1/4 will have the recessive trait. In humans, Brown hair is dominant to blond, so if two heterozygous people have children, each child has a 1 in 4 (1/4) chance of being blond.
For a dihybrid cross, you multiply the probabilities together. For example: curly hair is dominant to straight, so two people who are heterozygous for both brown hair and curly hair could get the following offspring:
Brown/Curly Brown/Straight Blond/Curly Blond/Straight 3/4 x 3/4 3/4 x 1/4 1/4 x 3/4 1/4 x 1/4 9/16 3/16 3/16 1/16
Incomplete dominance
Both alleles affect the phenotype. So homozygous dominant (red) looks different from heterozygous (pink).
Both alleles have capital letters eg CR ( red ), CW (white).
If two heterozygous individuals are crossed the offspring are in the ratio :
1 : 2 : 1 homozygous heterozygous homozygous dominant recessive (red) (pink) (white)
Polygenic inheritance
Polygenic inheritance means trait is affected by several genes. So there is a range of variation in the population. The offspring of heterozygous parents can be very different from either parent eg average sized parents can have a very tall child.
A graph of the expected offspring from heterozygous parents follows a normal distribution curve (bell curve). examples : skin color, height.
Genes are located on chromosomes; different species have different numbers of chromosomes, but about the same amount of DNA. Human & Mouse A typical human chromosome has roughly 1000 genes on it.
Linked genes are on the same chromosome. Linked genes tend to be inherited together. Linked genes are separated by crossing over in Prophase I. This gives different results from the Punnett square prediction.
A genetic map gives the location of genes, obtained by working out the frequency of crossing over. Only about 10% of a chromosome is genes, the rest has an unknown function ( originally called junk DNA), but a study in 2012 showed that 80% is active. Most consists of switches that turn genes on and off. In human DNA there may be as many as 4 million genetic switches.
Human cells have 22 pairs of non-sex ( autosomal ) chromosomes, that are the same in men and women, and 1 pair of sex chromosomes. The sex chromosomes are XX in the female and XY in the male.
X chromosomes are large (over 1000 genes). Y chromosomes are small (less than 80 genes)
Human Births: 52% male, 48% female because sperm carrying the Y chromosome are lighter and swim faster than ones with the X chromosome. Baby gender guarantee.
These diseases mainly affect men. They are caused by recessive genes on the X chromosome. Women have 2 copies of X, men have only 1 copy.
WOMEN | MEN | ||
---|---|---|---|
Genotype | Phenotype | Genotype | Phenotype |
XN XN | Normal | XN Y | Normal |
XN Xn | Carrier | Xn Y | Disease |
Xn Xn | Disease |
Sex-linked diseases are usually passed on from a carrier female to her son. Examples: colorblindness ( 8 % men colorblind, 0.5% women ). Test
Hemophilia ( blood does not clot ). Queen Victoria was a carrier. Royal disease
Barr bodies
Only one X chromosome is active in women, the other condenses to form the Barr body on the edge of the nucleus. The genes in the Barr body are inactive. Heterozygous females can have different X chromosomes active in different parts of the body eg Calico cats. Barr bodies are used to test for gender in sporting events like the Olympics, to make sure that female competitors really are female, and not men taking female hormones! Olympic sex tests
Genomic imprinting
Some genes have different effects depending on whether they are inherited from the mother or the father. These genes are imprinted, so some of the fathers genes are switched off in the sperm, and some of the mothers genes are switched off in the egg. If two sperm, or two eggs join, the embryo will not survive.
In mice, embryos with mainly the fathers genes have small heads, and large bodies. Embryos with mainly the mothers genes have small bodies and large heads. In mice, male imprinted genes affect lower parts of the brain ( feeding, reproduction, emotions ) whereas female imprinted genes affect the higher brain ( thinking, memory).
In humans, male imprinted genes increase the size of babies, female imprinted genes reduce the size.
Last edited June 2015 by David Byres, David.Byres@fscj.edu