Clinical Immunology and Serology by
"I love the way the book is organized."--Wendy Miller, MS, CLS(NSA), MT(ASCP)SI "I enthusiastically support the author's approach...I think this is a very valuable text..."--Stephen M. Johnson, MS, MT (ASCP) Completely updated to include new information about the immune system and new treatments for immunological diseases! This practical introduction to clinical immunology covers all of the essential theoretical principles and the serology techniques most commonly used in the laboratory. Structured into easy-to-read, student-friendly sections, the 3rd Edition focuses on the direct application of theory to clinical laboratory practice. With questions by Maribeth L. Flaws, Ph.D., SM(ASCP)SI, Rush University Medical Center, Chicago, Illinois and Christine Dorresteyn Stevens, EdD, MT(ASCP), Western Carolina University, Cullowhee, North Carolina
Manual of Clinical Microbiology by 
Clinical Microbiology for Diagnostic Laboratory Scientists by
A modern, evaluative, and integrative approach to diagnostic microbiology encouraging problem-solving in the clinical laboratory context through the use of examples to illustrate clinical and diagnostic issues Clinical Microbiology for Diagnostic Laboratory Scientists is designed to encourage readers to develop a way of thinking that can be applied to any diagnostic scenario in microbiology. Through consideration of a selected range of infections caused by pathogenic bacteria, viruses, fungi, protozoa, and helminths, the book encourages readers to explore connections between the available information about clinical symptoms, pathogenesis of infections, and the approaches used in laboratory diagnosis, in order to develop new insights. The book begins with an introductory chapter that outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of. The subsequent six chapters review a type of infection in depth, using particular pathogenic microorganisms to illustrate salient points. At the end of each chapter there are three exercises related to management of a diagnostic service and assessing the suitability of test methods to specific contexts. There are no right or wrong answers to these, but the reader can discuss them with their laboratory colleagues or university tutor. Makes extensive use of published research in the form of journal articles, publically available epidemiological data, professional guidelines, and specialist websites Stimulates the reader in critical appraisal of published evidence and encourages problem-solving in the laboratory Outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of Considers topics relevant to professional scientists working in the area of diagnostic microbiology Clinical Microbiology for Diagnostic Laboratory Scientists is ideal for post graduate scientists intending to pursue careers in diagnostic clinical microbiology and for biomedical scientists, clinical scientists, and full time students studying for upper level qualifications in biomedical science, microbiology, or virology.
Hematology and Coagulation by
Hematology and Coagulation is a clear and easy-to-read presentation of core topics and detailed case studies that illustrate the application of hematopathology knowledge to everyday patient care. In order to be successful, as well as to pass the American Board of Pathology examination, all pathology residents must have a good command of hematopathology, including the challenging topics of hematology and coagulation. Hematology and Coagulation meets this challenge head on. This basic primer offers practical examples of how things function in the hematopathology clinic as well as useful lists, sample questions, and a bullet-point format ideal for quick pre-board review. This book provides only the most clinically relevant examples designed to educate senior medical students, residents and fellows and "refresh" the knowledge base, without overwhelming students, residents, and clinicians. Takes a practical and easy-to-read approach to understanding hematology and coagulation at an appropriate level for both board preparation as well as a professional refresher course Covers all important clinical information found in larger textbooks in a more succinct and easy-to-understand manner Covers essential concepts in hematopathology in such a way that fellows and clinicians understand the methods without having to become specialists in the field
Hematology and Immunology by
This book is well written, concise, and easy to read and understand. It serves as a very handy and useful resource for busy laboratorians, who routinely encounter the situations detailed therein. It is also helpful for students, who need to learn how to recognize and avoid such situations, by providing expert guidance and examples of ways to keep these types of errors from potentially causing harm to patients.--Cynthia S. Johns, Laboratory Corporation of America, "Lab Medicine" The "Diagnostic Standards of Care" series presents common errors associated with diagnoses in clinical pathology, using case examples to illustrate effective analysis based on current evidence and standards. Each volume demonstrates the use of quality assurance and the role of the pathologist in ensuring quality and patient safety. "Hematology and Immunology" focuses on core issues in achieving quality in all areas of hematopathology and immunology, with an emphasis on identifying established, evidence-based standards. It addresses potential problems and sources of error in testing procedures, how to anticipate and avoid such problems, and how to manage them if they occur. Discussions are problem-based and address common situations and issues faced by clinical pathologists or members of a laboratory team. Using actual case studies, the book provides plentiful examples of errors, along with discussions on how to deal with them effectively."Hematology and Immunology Features"Key issues in achieving quality in all areas of hematology and immunologyNumerous case examples offering real-world illustrations of how problems occur and how to avoid themAn emphasis on identifying established, evidence-based standards in hematology and immunology
Immunohistochemistry: Basics and Methods by
establishedmethodsandeasy-to-adoptprotocols, payingattentiontomoderndev- opmentsinimmunohistochemistry, suchasantigenretrieval, signalampli'cation, the use of epitope tags in immunohistochemistry, multiple immunolabeling or diagnosticimmunohistochemistry. Eachofthemethodsdescribedinthishandbook v vi Preface was provedby the authors; many of these methods are routinely used in daily practiceintheirinstitute. Allthepracticalmethodsadvocatedareclearlydescribed, withaccompanyingtables, andtheresultsobtainableareillustratedwithcolour micrographs. Acknowledgements We thank Vera Samoilova forthe perfect technical assistance and other colleaguesfromtheMu]nsterUniversityClinicforsharingprobesandreagents. IgorB. BuchwalowandWernerBo]cker Mu]nster Contents 1 AntibodiesforImmunohistochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1. 1 StructureofAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1. 2 PolyclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1. 3 MouseMonoclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1. 4 RabbitMonoclonalAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1. 5 ProteinAandProteinGinImmunohistochemistry . . . . . . . . . . . . . . . . . . . 7 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2 AntibodyLabelingandtheChoiceoftheLabel . . . . . . . . . . . . . . . . . . . . . . . . 9 2. 1 CovalentLabelingofAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3 EnzymeLabelsforLightMicroscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy . . . . . . . . . . . . . . . . . . . 15 2. 5 ColloidalGoldLabelsforElectronMicroscopy . . . . . . . . . . . . . . . . . . . . . 16 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 3 ProbesProcessinginImmunohistochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3. 1 FixationinImmunohistochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3. 1. 1 FixationinAlcoholsandAcetone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 3. 1. 2 FixationinFormaldehyde . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 3. 1. 3 EffectofFormaldehydeFixationon Antigen AntibodyBinding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3. 2 Paraf'nSectionsforImmunohistochemicalAnalysis . . . . . . . . . . . . . . . 22 3. 2. 1 EmbeddingandCutting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 3. 2. 2 MountingParaf'nSectionsontoSlides . . . . . . . . . . . . . . . . . . . . . . . 23 3. 3 CryosectionsforImmunohistochemicalAnalysis . . . . . . . . . . . . . . . . . . . . 24 3. 4 BuffersforWashingandAntibodyDilution . . . . . . . . . . . . . . . . . . . . . . . . . 25 3. 5 MountingFollowingImmunohistochemicalStaining . . . . . . . . . . . . . . . . 27 3. 6 StorageFollowingImmunohistochemicalStaining . . . . . . . . . . . . . . . . . . 28 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 vii viii Contents 4 WorkingwithAntibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 4. 1 DirectImmunostainingMethod . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 4. 2 IndirectImmunostainingMethod . . . .
Laboratory Diagnostic Pathways by
The prognosis of a disease often depends on the timing of therapeutic invention, which in turn strongly relies on a reliable and quick diagnosis. Laboratory diagnostic pathways are algorithms that give structure to the diagnostic process, thereby minimizing the risk of mistreatment, shortening the hospital stay, and lowering the cost for treatment. This book offers 70 diagnostic algorithms that lead physicians and laboratory personnel through the diagnostic process in a step-by-step fashion. In Part One, general basics, infrastructure, and economic aspects are discussed and tipps for implementation are given. Part Two introduces screening methods for cases without a suspected diagnosis as well as specific pathways for stepwise diagnosis of the most common diseases, accompanied by information on pathophysiology, preanalytical measures, implementation, and interpretation of results.
Introduction to Diagnostic Microbiology for the Laboratory Sciences by
Introduction to Diagnostic Microbiology for the Laboratory Sciences provides a foundation in microbiology that is essential for a career as a medical laboratory technologist/technician (MLT). A key text for students and a helpful reference for practitioners, it reviews the microorganisms most commonly encountered in clinical settings and clearly explains basic laboratory procedures.This text provides a concise overview of topics and facilitates comprehension with learning objectives, key terms, case studies, and review questions. In addition, the text includes laboratory exercises, eliminating the need for a separate laboratory manual. Covering content required in the MLT curriculum and featured on the certification exam, this accessible text will help prepare students for a career in laboratory science.Key Features* Reviews the microorganisms most important in clinical practice* Explains basic laboratory procedures, such as specimen collection and staining * Includes laboratory exercises in the text-no need for a separate manual* Serves as a helpful on-the-job reference for laboratory practitioners* Provides practice questions to help students prepare for the medical technology certification examCHAPTER PEDAGOGY: Chapter Outline, Key Terms, Learning Objectives, Procedures, Laboratory Exercises, Case Studies, Review QuestionsINSTRUCTOR RESOURCES: Image Bank with 247 photos and illustrations; PowerPoint Presentations per chapter; Laboratory Exercise Worksheets; and a Test Bank with 450 multiple choice questions and a 225-question exam.Introduction to Diagnostic Microbiology for the Laboratory Sciences is on the recommended reading list to prepare for the ASCP MLT exam. (American Society for Clinical Pathology, Medical Laboratory Technician exam)
Fundamental Laboratory Mathematics by